R & D Laser Lab

Glass Scribing Laser Solution

June 3, 2019

Glass micro slides and Graticule slides

Laser solution marking for glass and glass micro slides made exactly of fused quartz or either borosilicate glass are used for characterization and sampling purposes. Plain slides are often used for holding liquid or powder samples under microscopy devices. The thickness of the slide varies according to the ultraviolet transparency requirement (0.2 mm-2 mm). Slides may have rounded corners for increased safety or robustness or they may have a cut-off corner for use with a slide clamp or cross-table. The edges are cut out using a diamond cutter most of the time; but lately, manufacturers are opting for more of precise-swift options like a laser for scribing their material out, for a smoother edge formation.

Laser marking for Glass

Graticule slides are marked with a grid of lines (for example, a 1 mm grid) which then allows the size of objects seen under microscopic magnification to be easily estimated and provides researchers with a reference area for counting minute objects. Sometimes, one square of the grid will itself be subdivided into a finer grid. The scale or grid is centered on a glass disc mounted in a black anodized aluminum slide and mostly used as calibration and resolution checking of microscopes and image analysis systems. The combination of scales, dots, circles, squares, rulings, grids and angles can be supplied with an internationally traceable certificate of calibration for those who require ISO conformity.

Laser Processing

The microslide blanks are processed with Markolaserâ„¢ low wattage, frequency doubled laser source for the scribing purpose under a closed environment; providing enough air pocket under the processing stage. The sharp-cornered and round-cornered shapes are scribed out from the slide using high pulse energy of the laser. The thickness of the glass is around 0.3mm.

The Microslide Blanks are processed with Markolaser

While, the grid marking of the graticule slides of 2 mm thickness at the center of the thickness is performed with Markolaserâ„¢ tripled frequency laser source; creating sharp micro-cracking of the cells within the glass to form a regular grid pattern of size 3×3 mm. The pulse energy is kept lower to avoid cracking of the glass more than it’s required.


The scribing and grid marking results are captured with a 200x microscope. The scribing shows spectacular edge patterns with no signs of thermal cracking.

Laser solution sample

 sharp cornered scribing
Sharp cornered scribing
round scribing laser cutting
Round shaped scribing

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